翻訳と辞書 Words near each other ・ Pair-et-Grandrupt ・ Pair-instability supernova ・ Pair-rule gene ・ Paira ・ Pairagachha ・ Pairan ・ Pairat Decharin ・ Pairatahi River ・ Pairc nan Laoch ・ Paired data ・ Paired difference test ・ Paired disparity code ・ Paired home ・ Paired metamorphic belts ・ Paired opposites ・ Paired-end tag ・ Pairi Daiza ・ Pairi River ・ Pairie ・ Pairing ・ Pairing (computing) ・ Pairing function ・ Pairing heap ・ Pairing Off ・ Pairing-based cryptography ・ Pairis Abbey ・ Pairoj Borwonwatanadilok ・ Pairote Pongjan ・ Pairote Sokam ・ PAIRS Foundation Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Paired-end tag ： ウィキペディア英語版 Paired-end tag Paired-end tags (PET) (sometimes "Paired-End diTags", or simply "ditags") are the short sequences at the 5’ and 3’ ends of a DNA fragment which are unique enough that they (theoretically) exist together only once in a genome, therefore making the sequence of the DNA in between them available upon search (if full-genome sequence data is available) or upon further sequencing (since tag sites are unique enough to serve as primer annealing sites). Paired-end tags (PET) exist in PET libraries with the intervening DNA absent, that is, a PET "represents" a larger fragment of genomic or cDNA by consisting of a short 5' linker sequence, a short 5' sequence tag, a short 3' sequence tag, and a short 3' linker sequence. It was shown conceptually that 13 base pairs are sufficient to map tags uniquely.〔Fullwood MJ, Wei CL, Liu ET, Ruan Y. 2009. Next-Generation DNA sequencing of paired-end tags (PET) for transcriptome and genome analyses. Genome Research. 19:521–532. PMID 19339662〕 However, longer sequences are more practical for mapping reads uniquely. The endonucleases (discussed below) used to produce PETs give longer tags (18/20 base pairs and 25/27 base pairs) but sequences of 50–100 base pairs would be optimal for both mapping and cost efficiency.〔 After extracting the PETs from many DNA fragments, they are linked (concatenated) together for efficient sequencing. On average, 20–30 tags could be sequenced with the Sanger method, which has a longer read length.〔 Since the tag sequences are short, individual PETs are well suited for next-generation sequencing that has short read lengths and higher throughput. The main advantages of PET sequencing are its reduced cost by sequencing only short fragments, detection of structural variants in the genome, and increased specificity when aligning back to the genome compared to single tags, which involves only one end of the DNA fragment. ==Constructing the PET library== PET libraries are typically prepared in two general methods: cloning based and cloning-free based. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Paired-end tag」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.